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Updated: Aug 13, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
Multifunctionality of human 17beta-hydroxysteroid dehydrogenases
Gabriele Moeller1, Jerzy Adamski
1GSF-National Research Center of Environment and Health, Institute of Experimental Genetics, Genome Analysis Center, Neuherberg, Germany. gabriele.moeller@gsf.de
Abstract:
17Beta-hydroxysteroid dehydrogenases (17beta-HSDs) belong to the family of short chain dehydrogenases/reductases (SDRs) and aldoketo-reductases (AKRs). Some of the enzymes were discovered and named due to their enzymatic activity on steroid substrates or according to their sequence homology to other 17beta-HSDs. During characterisation of these enzymes it turned out that their substrate specificity is broader than first expected and key functions of some 17beta-HSDs in vivo are probably not in steroid metabolism but in basic metabolic pathways. The issue of such multifunctionality is the topic of this review.
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