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Protein depletion from blood plasma using a volatile buffer
Dmitri Sitnikov1, Donovan Chan, Eric Thibaudeau
1Caprion Pharmaceuticals, Inc., Department of Protein Analysis, 7150 Alexander Fleming, Montreal, Que., Canada H4S-2C8. dsitnikov@caprion.com
Summary
This study introduces a new immunoaffinity (IA) depletion method for blood plasma proteomics. It uses a volatile buffer, simplifying sample processing and improving efficiency for high-abundance protein removal.
Area of Science:
- Proteomics
- Biochemistry
- Analytical Chemistry
Background:
- High-abundance protein removal is crucial for blood plasma proteomics.
- Immunoaffinity (IA) depletion is the most specific method but traditionally requires inorganic buffers.
- Post-depletion desalting steps reduce sample integrity and increase costs.
Purpose of the Study:
- To develop an improved IA depletion method for blood plasma.
- To eliminate the need for post-depletion desalting steps.
- To enhance reproducibility and efficiency in plasma sample processing.
Main Methods:
- Developed a novel IA depletion technique utilizing a volatile buffer system.
- Implemented lyophilization to remove the volatile buffer post-depletion.
- Enabled semi-automated execution of the depletion process.
Main Results:
- Successfully removed high-abundance proteins from blood plasma.
- Eliminated the need for traditional desalting procedures.
- Demonstrated reproducible and efficient depletion with the new method.
Conclusions:
- The volatile buffer IA depletion method streamlines plasma proteomics workflows.
- This approach preserves sample integrity and reduces processing time and cost.
- Offers a more efficient and robust solution for high-abundance protein removal in proteomics.
