Related Experiment Video
Updated: Aug 13, 2026

Assaying for Inorganic Polyphosphate in Bacteria
Published on: January 21, 2019
Effect of boiling on dietary phosphate and nitrogen intake
Adamasco Cupisti1, Francesco Comar, Omar Benini
1Department of Internal Medicine, Nephrology, University of Pisa, Pisa, Italy. acupisti@med.unipi.it
Objective:
Dietary phosphate restriction is one of the means of phosphatemia control in dialysis patients. To limit dietary phosphate intake, appropriate food choices are recommended, but this often creates a conflict with the high-normal protein requirement of dialysis patients. Although food processing by boiling may be a safe tool for eliminating many minerals, this method poses a risk for loss of important nutrients, including proteins. The goal of this study was to assess the effect of boiling on phosphate and protein nitrogen changes in commonly used foods that contain proteins of high biological value.
Methods:
We evaluated the true retention values of dry matter, crude protein, and total phosphorus in fresh beef and chicken breast before and after 10, 20, and 30 minutes of boiling; the reported values represent the average of five determinations.
Results:
Compared with crude raw samples, dry matter retention in cooked beef was reduced up to 92% +/- 6%, crude protein retention was reduced up to 87% +/- 10%, and phosphorous retention was reduced up to 42% +/- 13%; similar data were obtained when boiling the chicken breast, 93% +/- 3%, 81% +/- 4%, and 63% +/- 6%, respectively.
Conclusions:
Our results show that consuming boiled foods can significantly reduce dietary phosphate while preserving protein intake, namely reducing the effective phosphate intake per gram of dietary protein. This can represent additional advice to the patient for limiting the dietary phosphorus load at the same protein intake, leading to a better control of phosphate balance together with a lower risk of protein malnutrition.
More Related Videos
Related Concept Videos
Introduction to Electrolytes
Role of Sodium
One...
Phosphorylation
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Roles of Electrolytes: Calcium and Phosphate
The calcium concentration in blood plasma is primarily regulated...
Other Nuclides: 31P, 19F, 15N NMR
While fluorine-19 and phosphorous-31 have high natural abundances (100%) and positive gyromagnetic ratios, nitrogen-15 has a low natural abundance and a negative gyromagnetic ratio. However, nitrogen-15 is still preferred over nitrogen-14 (which has a high...
2° Amines to N-Nitrosamines: Reaction with NaNO2
Hormones and Bone Tissue
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...

