Related Experiment Video
Updated: Jan 11, 2026

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
Published on: June 5, 2020
[Dynamic expression of hepatitis B virus covalently closed circular DNA in 2.2.15 cell]
Mao-chang Liu1, Gui-qiang Wang, Wen-hua Piao
1Department of Infectious Diseases, Peking University First Hospital, Beijing 100034, China.
Background:
To determine the presence of covalently closed circular DNA (cccDNA), and to investigate the expression kinetics of HBV DNA, HBsAg and HBeAg in 2.2.15 cell.
Methods:
HBV cccDNA was assessed by polymerase chain reaction, HBV DNA was measured by Taqman quantitative PCR and HBsAg and HBeAg was measured by EIA.
Results:
HBV cccDNA was found in both intracellular and extracellular space. There was a good correlation between HBsAg, HBeAg and HBV DNA in the supernatant of 2.2.15 cell (r= 0.833, P < 0.05 and r= 0.939, P < 0.01 for HBsAg and HBeAg, respectively), whereas there was no significant correlation between intracellular HBV DNA levels and virus antigen levels (r= 0.024, P= 0.955 and r= 0.177; P= 0.625 for HBsAg and HBeAg, respectively).
Conclusion:
HBV cccDNA was detectable in the culture medium and intracellularly in 2.2.15 cells, and these data provided an indication of HBV replication in 2.2.15 cell.
Related Concept Videos
Viruses with RNA Genomes
DNA Bacteriophages
Size and Structure of Viral Genomes
Retrovirus Life Cycles
Retroviruses

