Related Experiment Video
Updated: Aug 13, 2026
Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates
Published on: January 27, 2019
Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different
D A D'Avila1, E D Gontijo, E Lages-Silva
1Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 Caixa Postal 486, 31270-901, Belo Horizonte, Minas Gerais, Brazil. galvao@icb.ufmg.br
Abstract:
The genetic variability of 61 Trypanosoma cruzi isolates from 47 chronic chagasic patients of Minas Gerais state was analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using M13-40, lambdagt11-F, and L15996 primers. Cluster analysis by unweighted pair group method analysis was applied to RAPD profiles, and cluster analysis used to verify a possible correlation among different clinical forms of the disease from these patients. The T. cruzi isolates showed distinct grouping on tree topology, with the isolates not being possible to establish a correlation to the clinical forms of Chagas' disease. These data showed that the T. cruzi isolates from these patients would compose a group of populations well correlated genetically.
