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Updated: Aug 13, 2026

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
Published on: June 2, 2022
Calcium intake, food sources and seasonal variations in eastern Croatia
Mirna Mandić-Puljek1, Milena Lela Mandić, Antonija Perl
1University Hospital for Pulmonary Diseases "Jordanovac", Zagreb, Croatia.
Abstract:
The objective of this study was to examine the quantity of calcium intake among adults, the sources of calcium, differences among seasons, as well as the differences between sexes, correlation with body mass index (BMI), and age. The study included 161 healthy volunteers from the eastern part of Croatia. Each subject completed three food frequency questionnaires (FFQ) with 150 items, at an interval of 3-4 months. The mean calcium intake for whole population for all three FFQs was 965 mg/day. At the same time, the quantity of calcium for all subjects was 14.2 mg/kg, women 14.5 mg/kg, and men 13.6 mg/kg, respectively. There was an inverse relation between calcium intake and age for men (r = -0.32 p = 0.028), but not for women. Correlation between calcium intake and BMI was negative, but not significantly. Milk and dairy products were the main source of calcium. Marginally low mean calcium intake goes to show the needful to educate the population.
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