Convergent oxygenation of arachidonic acid by 5-lipoxygenase and cyclooxygenase-2
Claus Schneider1, William E Boeglin, Huiyong Yin
1Department of Pharmacology, Division of Clinical Pharmacology, 23rd Avenue South at Pierce, Vanderbilt University Medical School, Nashville, Tennessee 37232, USA. claus.schneider@vanderbilt.edu
Abstract:
5-Lipoxygenase and cyclooxygenase-2 (COX-2) initiate the biosynthesis of distinct families of mediators from arachidonic acid (leukotrienes and prostaglandins, respectively) and are each the target of anti-inflammatory medications. Here we show that the product of 5-lipoxygenase, 5S-hydroxyeicosatetraenoic acid, is selectively and efficiently triply oxygenated by COX-2, implicating a cross-pathway interaction. The product is a unique diendoperoxide, potentially representing the parent compound of a novel group of lipid mediators.
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