Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic complementation to identify DNA elements that influence complement resistance in Leishmania chagasi.

R R Dahlin-Laborde1, T P Yu, J K Beetham

  • 1Department of Entomology, Iowa State University, Ames 50011-1250, USA.

The Journal of Parasitology
|January 20, 2006
PubMed
Summary

Researchers identified new factors that help Leishmania chagasi parasites resist complement-mediated lysis (CML). This discovery advances understanding of parasite survival mechanisms and potential therapeutic targets.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[IDH-mutant adult-type diffuse gliomas: a clinicopathological analysis of 1 301 cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

Generation of attosecond electron bunches of tunable duration and density by relativistic vortex lasers in near-critical density plasma.

Optics express·2024
Same author

[Pilocytic astrocytoma with KRAS gene mutation: a clinicopathological analysis of two cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2024
Same author

Effect of metformin on Wnt5a in individuals new-onset type 2 diabetes with different body mass indexes: The evidences from the real word research.

Journal of diabetes and metabolic disorders·2023
Same author

[Cardiac amyloidosis: pathological classification and clinical analysis of 48 cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2023
Same author

Effect of glucagon-like peptide-1 receptor agonist on insulin secretion index and serum Wnt5a protein in patients with new-onset type 2 diabetes mellitus.

Journal of diabetes and metabolic disorders·2023

Area of Science:

  • Parasitology
  • Immunology
  • Molecular Biology

Background:

  • Leishmania spp. promastigotes show varying sensitivity to complement-mediated lysis (CML) during different growth phases and culture conditions.
  • Leishmania chagasi exhibits CML resistance in stationary phase cultures from infected animals, but becomes constitutively sensitive after serial passaging.

Purpose of the Study:

  • To identify novel genetic factors conferring complement resistance in Leishmania chagasi.
  • To understand the molecular mechanisms behind Leishmania's survival against host immune defenses.

Main Methods:

  • A genetic screen using a cosmid library was performed on a serially passaged, CML-sensitive L. chagasi promastigote line.
  • Transfection of promastigotes with the library and selection for CML resistance using human serum.

Related Experiment Videos

  • Isolation and sequencing of cosmids conferring resistance to identify candidate genes.

    • Main Results:

    • Twelve transfectant clones resistant to CML were obtained.
    • Cosmids from 7 clones conferred significant CML resistance (37% survival) when transfected into a naive CML-sensitive line.
    • Sequencing of one cosmid revealed an insert with 89% identity to a region on Leishmania major chromosome 36, encoding 6 hypothetical proteins.

    Conclusions:

    • Novel complement resistance factors in L. chagasi have been identified through a genetic screen.
    • The identified genes, homologous to those on L. major chromosome 36, are potential targets for understanding and combating Leishmania infections.