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A minimalist design approach to antimicrobial agents based on a thionin template
Miquel Vila-Perelló1, Sabrina Tognon, Andrea Sánchez-Vallet
1Department of Experimental and Health Sciences, Pompeu Fabra University, E-08003 Barcelona, Spain.
Abstract:
Numerous studies have been devoted to the stabilization of secondary structure elements to improve receptor-ligand recognition. We report a novel application of this principle to create new antimicrobial agents using the highly folded thionin from Pyrularia puberaas a template. Non-native disulfide bonds have been used to induce two short linear segments of the protein into an amphipathic helix. The resulting 13- and 9-residue peptides are significantly more active than their linear counterparts and have an activity similar to that of native thionin.
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