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Related Concept Videos

Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Related Experiment Video

Updated: Jul 14, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

Discovery of Time-Delayed Gene Regulatory Networks based on temporal gene expression profiling.

Xia Li1, Shaoqi Rao, Wei Jiang

  • 1Department of Bioinformatics, Harbin Medical University, Harbin 150086, PR China. lixia@ems.hrbmu.edu.cn

BMC Bioinformatics
|January 20, 2006
PubMed
Summary

We developed TdGRN, a novel computational toolbox, to reconstruct time-delayed gene regulatory networks from temporal gene expression data. This method successfully identified key gene regulations in yeast and human cell cycles.

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Last Updated: Jul 14, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
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Area of Science:

  • Systems Biology
  • Bioinformatics
  • Genomics

Background:

  • Elucidating molecular determinants of life phenomena like cell cycling and disease pathogenesis is crucial.
  • Large-scale, time-resolved genomic data offers opportunities to study time-delayed gene regulatory networks.

Purpose of the Study:

  • To develop a novel computational method for reconstructing gene regulatory networks from temporal gene expression data.
  • To address the challenge of uncovering time-delayed gene interactions across various time scales.

Main Methods:

  • Developed a model-free computational toolbox named TdGRN (Time-delayed Gene Regulatory Network).
  • Utilized delayed correlations and a time-delayed gene expression matrix for network reconstruction.
  • Applied the method to yeast and human HeLa cell cycle datasets.

Main Results:

  • Successfully reconstructed time-delayed gene regulatory networks for cell cycling.
  • Identified numerous gene regulations supported by experimental evidence.
  • Demonstrated consistency with known cell cycle phase characteristics.

Conclusions:

  • Established a powerful, model-free approach for dissecting dynamic gene-gene interactions.
  • Validated the TdGRN algorithm using public cell cycling datasets.
  • The approach is applicable to studying gene regulation in development, aging, and complex diseases.