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Updated: Aug 13, 2026

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
Published on: July 16, 2012
[Inhibition of HCV replication by HCV specific U1 small nuclear RNA chimeric ribozyme in vivo]
Mei-xia Wang1, Qing-long Jin, Yu Pan
1Department of Infectious Diseases, First Hospital of Jilin University, Changchun 130021, China. wangmeixiad@163.com
Objective:
To determine the advantage of U1 small nuclear RNA as a ribozyme vector (U1-Rz) to inhibit HCV replication in vivo.
Methods:
The 3rd stem-loop was substituted by HCV core specific ribozyme to construct an U1-Rz eucaryotic expression plasmid. Then it was co-transfected with pCMV/T7-NCRC Delta-luc into Huh7 cell line mediated by lipofectin. The cell lysis supernatant was subjected to Western blot and lumenometer to determine the luciferase levels.
Results:
A U1 snRNA chimeric ribozyme was constructed successfully. Both Rz and U1-Rz inhibited luciferase expression in Huh7 by 48.64% and 87.46%, respectively.
Conclusion:
Rz has more efficacy in cells when using U1 snRNA delivery system. U1 can be an efficient vector for HCV specific ribozyme.
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