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Subchronic rat inhalation study with Skydrol 500B-4 fire resistant hydraulic fluid
C E Healy1, R S Nair, W E Ribelin
1Monsanto Company, St. Louis, MO 63167.
American Industrial Hygiene Association Journal
|March 1, 1992
Summary
Skydrol 500B-4, a hydraulic fluid, caused irritation and some toxicity in rats. High exposure led to reduced body weight, altered liver weights, and decreased red blood cell counts in female rats.
Area of Science:
- Toxicology
- Occupational Health
Background:
- Skydrol 500B-4 is a fire-resistant hydraulic fluid used in commercial aviation.
- Phosphate ester mixtures like dibutyl phenyl phosphate (DBPP) and tributyl phosphate (TBP) are key components.
Purpose of the Study:
- To evaluate the inhalation toxicity of Skydrol 500B-4 in Sprague-Dawley rats.
- To identify potential health risks associated with exposure to this hydraulic fluid.
Main Methods:
- Rats were exposed to Skydrol 500B-4 at concentrations of 0, 5, 100, and 300 mg/m³ for 6 hours/day, 5 days/week.
- Particle size analysis confirmed respirable fractions.
- Animals were assessed after 6 and 13 weeks of exposure.
Main Results:
- A reddish nasal discharge and oral salivation were observed in mid- and high-exposure groups, indicating an irritant response.
- High-exposure female rats showed statistically significant reduced body weights, increased liver weights, and decreased erythrocyte counts, hemoglobin, and hematocrit after 13 weeks.
- High-exposure male rats exhibited increased liver weights and decreased hematocrit after 13 weeks. Plasma cholinesterase was reduced in high-exposure females.
Conclusions:
- Skydrol 500B-4 exhibits irritant properties upon inhalation.
- Prolonged high-level exposure can lead to systemic toxicity, particularly affecting hematological parameters and liver weight in female rats.