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Assay and purification of calmodulin-dependent cyclic nucleotide phosphodiesterase and isozyme separation

Rajendra K Sharma1

  • 1Department of Pathology, Saskatoon Cancer Centre, College of Medicine, University of Saskatchewan, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|January 21, 2006
PubMed
Abstract

No abstract available in PubMed .

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Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...