[Determination of hedychenone in Hedychium yunnanense]
Qi-xin Yan1, Xiao-wen Ye, Qing Zhao
1School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510000, China. yanqixin@eyou.com
Objective:
To establish an HPLC method for determination of hedychenone in Hedychium yunnanense.
Method:
C18 Chromatographic column was used, acetonitrile-water (9:1) as mobile phase, at flow rate of 1.0 mL x min(-1): The wavelength for detection was 235 nm.
Result:
The linear range of hedychenone was 5.92-29.6 microg x mL(-1)(r = 0.9999). The average recovery was 99.0%, RSD of precision was less than 2%.
Conclusion:
The method is simple, effective and feasible, and can be used to evaluate the quality of the herb.
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