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[Effect of Kanglaite injection oncyclooxygenase activity in lung carcinoma A549 cell]
Qing-hua Dong1, Xian Zhong, Shu Zheng
1The 2nd Affiliated Hospital of Zhejiang University, Cancer Institute, Zhejiang University, Hangzhou 310016, China.
Objective:
To study the effect of Kanglaite injection on cyclooxygenase activity in lung carcinoma A549 cells.
Methods:
The expression of mRNA of COX-1 and COX-2 were measured by RT-PCR. The expression of COX-2 protein was measured by Western-blot.
Result:
Kanglaite injection could selectively decreased COX-2 mRNA expression and protein expression while COX-1 mRNA expression was unchanged.
Conclusion:
Kanglaite injection is selectively inhibitory agent of COX-2, and possess inhibitory effects on cyclooxygenase 2.
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