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Updated: Aug 13, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Lives and times of nuclear receptors

Elaine T Alarid1

  • 1Department of Physiology, University of Wisconsin-Madison, 120 Service Memorial Institute, 1300 University Avenue, Madison, Wisconsin 53706, USA. alarid@physiology.wisc.edu

Molecular Endocrinology (Baltimore, Md.)
|January 21, 2006
PubMed

Insights

Ligand-induced receptor down-regulation, initially termed processing, involves proteolysis by the 26S proteasome. This mechanism is crucial for transcriptional activation and nuclear receptor function.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Biochemistry

Background:

  • Steroid hormone action was early characterized by ligand-induced receptor down-regulation, termed processing.
  • Early studies noted a correlation between receptor turnover and gene transcription induction.

Purpose of the Study:

  • To review the mechanism and relevance of proteolysis in nuclear receptor function.
  • To explore the link between ligand-induced receptor processing and transcriptional activation.

Main Methods:

  • Review of existing literature on nuclear receptor family analyses.
  • Examination of the role of the 26S proteasome in receptor processing.

Main Results:

  • Ligand-induced receptor processing involves proteolysis mediated by the 26S proteasome.
  • The 26S proteasome's activity is linked to cell-cycle control, signal transduction, and transcription.

Conclusions:

  • Proteolysis is a key mechanism in nuclear receptor function and transcriptional regulation.
  • Understanding proteolysis offers renewed insight into steroid hormone action and gene transcription.

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