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Related Experiment Videos

Opioids trigger alpha 5 beta 1 integrin-mediated monocyte adhesion.

Oscar M Pello1, Béatrice Duthey, David García-Bernal

  • 1Department of Immunology and Oncology, Centro Nacional de Biotecnología/Consejo Superior de Investigaciones Científicas, Madrid, Spain.

Journal of Immunology (Baltimore, Md. : 1950)
|January 21, 2006
PubMed
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Opioids modulate immune cell function by triggering monocyte adhesion via specific receptors and signaling pathways. This discovery reveals a new role for opioids in regulating immune cell movement and inflammation.

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Inflammatory responses involve complex signaling networks.
  • Immune cells produce opioid peptides in inflamed tissues, contributing to analgesia.
  • Opioid peptides act on peripheral sensory neurons for pain relief.

Purpose of the Study:

  • To investigate the role of opioids in modulating immune cell function.
  • To elucidate the mechanisms by which opioids influence immune cell adhesion and trafficking.

Main Methods:

  • Utilized DPDPE, an opioid receptor-specific ligand, to trigger monocyte adhesion in vitro and in vivo.
  • Employed neutralizing anti-alpha5beta1 integrin monoclonal antibodies (mAbs) to assess integrin involvement.
  • Investigated signaling pathways including PI3Kgamma, Src kinases, Vav-1, Rac1, and G proteins using pertussis toxin treatment.

Related Experiment Videos

Main Results:

  • DPDPE binding to its receptor initiated monocyte adhesion.
  • Adhesion was dependent on alpha5beta1 integrin, PI3Kgamma activation, Src kinases, Vav-1, and Rac1.
  • DPDPE induced adhesion in pertussis toxin-treated cells, suggesting involvement of non-Gi G proteins.

Conclusions:

  • Opioids play a significant role in regulating leukocyte trafficking beyond their known analgesic effects.
  • This study identifies a novel function for opioids in modulating immune cell adhesion and inflammatory responses.
  • Findings highlight the intricate interplay between opioid signaling and immune cell behavior.