Related Experiment Video
Updated: Aug 13, 2026
Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
Published on: May 18, 2022
RCAS-RNAi: a loss-of-function method for the developing chick retina
Sanjiv Harpavat1, Constance L Cepko
1Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, USA. sanjiv_harpavat@student.hms.harvard.edu
Background:
The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function.
Results:
We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection.
Conclusion:
This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina.
