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Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke
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Thrombin in ischemic neuronal death

Marlise de Castro Ribeiro1, Jérôme Badaut, Melanie Price

  • 1Neurology Service, BH 19 208, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.

Experimental Neurology
|January 24, 2006
PubMed

Thrombin plays a role in cerebral ischemia as rats subjected to focal cerebral ischemia were protected by the intracerebral injection of hirudin, a selective thrombin inhibitor. To separate the roles of thrombin in cell death and in coagulation, we have used an in vitro approach to test the effect of hirudin and of protease nexin-1 (PN-1), a cerebral thrombin inhibitor, on neuronal ischemia. Rat organotypic hippocampal slice cultures were subjected to oxygen (5%) and glucose (1 mmol/L) deprivation (OGD) during 30 min. Hirudin or PN-1 administered after OGD significantly prevented neuronal death in the CA1 region. After 24 h, there was a marked increase in thrombin immunoreactivity on Western blots. Thrombin therefore contributes to ischemic damage in neural tissue in vitro.

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