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Published on: June 20, 2019
Ferulic acid esterase production by Streptomyces sp
G Mukherjee1, R K Singh, A Mitra
1Microbiology Division, School of Life Science, Visva-Bharati, Santiniketan 731 235, India.
Bioresource Technology
|January 24, 2006
Summary
Researchers optimized ferulic acid esterase production using Streptomyces S10. The best enzyme yield reached 2.0 mU/ml under specific agitated submerged culture conditions.
Area of Science:
- Biochemistry
- Microbiology
- Enzymology
Background:
- Ferulic acid esterase is crucial for breaking down plant cell walls.
- Streptomyces species are known producers of valuable industrial enzymes.
- Optimizing enzyme production is key for cost-effective industrial applications.
Purpose of the Study:
- To optimize the production of ferulic acid esterase by Streptomyces S10.
- To determine the ideal conditions for maximizing enzyme yield.
Main Methods:
- Cultivation of Streptomyces S10 in MBS medium.
- Utilizing de-starched wheat bran as a substrate.
- Employing agitated submerged culture techniques.
- Testing various temperature and pH parameters.
Main Results:
- Achieved an enzyme yield of 2.0 mU/ml under optimized conditions.
- Identified optimal temperature at 30°C and initial pH at 6.5.
- 1.5% de-starched wheat bran was found to be the optimal substrate concentration.
Conclusions:
- Ferulic acid esterase production by Streptomyces S10 can be significantly enhanced through optimized submerged culture.
- The study provides a foundation for large-scale production of this enzyme for biotechnological purposes.
