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Updated: Aug 13, 2026
Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
Published on: June 29, 2018
Sensitive and specific detection of Xanthomonas axonopodis pv. citri by PCR using pathovar specific primers based on
Dong Suk Park1, Jae Wook Hyun, Young Jin Park
1National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea. dspark@rda.go.kr
Abstract:
A sensitive and specific assay was developed to detect citrus bacterial canker caused by Xanthomonas axonopodis pv. citri, in leaves and fruits of citrus. Primers XACF and XACR from hrpW homologous to pectate lyase, modifying the structure of pectin in plants, were used to amplify a 561 bp DNA fragment. PCR technique was applied to detect the pathogen in naturally or artificially infected leaves of citrus. The PCR product was only produced from X. axonopodis pv. citri among 26 isolates of Xanthomonas strains, Escherichia coli (O157:H7), Pectobacterium carotovorum subsp. carotovorum, and other reference microbes.
