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Herpes simplex virus type 1 recombinants without the oriL sequence replicate DNA with increased fidelity
Changying Jiang1, Ying T Hwang, Charles Bih-Chen Hwang
1Department of Microbiology and Immunology, State University of New York, Upstate Medical University, 750 E. Adams St. Syracuse, NY 13210, USA.
Virology
|January 24, 2006
Summary
Herpes simplex virus type 1 (HSV-1) DNA replication fidelity increased in viruses lacking the oriL origin. This suggests alternative mechanisms enhance accuracy in HSV-1 DNA replication without intact oriL sequences.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Herpes simplex virus type 1 (HSV-1) possesses three DNA replication origins (ori): one oriL and two oriS.
- oriL is centrally located in unique long sequences, while oriS copies are in reiterated sequences flanking unique short sequences.
Purpose of the Study:
- To investigate the role of oriL in HSV-1 DNA replication fidelity.
- To assess the impact of oriL deletion on gene replication accuracy.
Main Methods:
- Construction of recombinant HSV-1 (ts+7) with oriL deletion.
- Integration of reporter genes (beta-galactosidase or supF amplicon) into the thymidine kinase locus.
- Mutagenesis assays on recombinant viruses and rescue recombinants.
Main Results:
- HSV-1 viruses with deleted oriL sequences exhibited higher fidelity in replicating target genes compared to the parental strain (KOS).
- Recombinant viruses lacking intact oriL sequences showed enhanced DNA replication accuracy.
Conclusions:
- The absence of intact oriL sequences in HSV-1 may lead to increased DNA replication fidelity.
- Potential mechanisms for high-fidelity DNA replication in oriL-deficient HSV-1 warrant further investigation.