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Detection of Mycobacterium tuberculosis complex with Real Time PCR: comparison of different primer-probe sets based

Paul H M Savelkoul1, Arnold Catsburg, Sije Mulder

  • 1Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands. p.savelkoul@vumc.nl

Journal of Microbiological Methods
|January 24, 2006
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...