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Updated: Aug 13, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
A bacteroides conjugative transposon, CTnERL, can transfer a portion of itself by conjugation without excising from
Gabrielle Whittle1, Nathan Hamburger, Nadja B Shoemaker
1Department of Microbiology, 601 S. Goodwin Ave., University of Illinois, Urbana, IL 61801, USA.
Abstract:
CTnERL, a Bacteroides conjugative transposon, transferred DNA by an Hfr-type mechanism during conjugation when it was excision deficient due to an insertion in the integrase gene. Rescue of the conjugative transposon sequences required the recipient to be RecA proficient and to contain an integrated CTnERL. The transfer efficiency was only 10- to 30-fold lower than the normal element transfer efficiency, and the direction of transfer from the oriT gene showed that the integrase end was transferred first and that the transfer genes were transferred last.
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