The differential antibacterial and gastrointestinal effects of erythromycin and its chiral isolates
Ivana Cvetanovic1, Vasant Ranade, Congrong Lin
1Department of Pharmacology, Rush University, Chicago, Illinois, USA.
Abstract:
The use of erythromycin has been limited by the gastrointestinal side effect properties, which include abdominal distress and diarrhea. To evaluate the possibility of reducing the toxicity of erythromycin, studies were undertaken to separate erythromycin into chiral isolates and then to test the activity of these chiral isolates on gastrointestinal contractility and bacteriostatic actions. Gastrointestinal contractility was obtained by the use of isolated strips of a rat colon. Antibacterial activity was used by obtaining the MICs of erythromycin and isolated agents against Enterococcus faecalis ATCC 29212. ANOVA was performed using the SPSS v.10 to determine statistical differences in the MICs and the amplitude and frequency of spike bursts. Results were expressed as mean+/-SE (N=5). The MICs (microg/mL) of erythromycin (racemate), chiral isolate X, and chiral isolate Y were 0.45+/-0.29, 0.53+/-0.24 (n.s.), and 0.2+/-0.07 (P
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