Related Experiment Video
Updated: Aug 13, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Sequential injection analysis for the simultaneous determination of clavulanic acid and amoxicillin in
1Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Marcel-lí Domingo s/n (Campus Sescelades), 43007 Tarragona, Spain. alberto.pasamontes@urv.net
Abstract:
In this paper, we report on a method for quantifying clavulanic acid and amoxicillin simultaneously in pharmaceuticals using sequential injection analysis (SIA) with a diode-array spectrophotometric detector and multivariate curve resolution with alternating least squares (MCR-ALS). We optimized the experimental parameters so that the analytical sequence could distinguish the concentrations and spectrum profiles of the species of interest with optimum resolution quality. After establishing the optimum conditions, we quantified clavulanic acid and amoxicillin in four pharmaceuticals. In most cases our results were slightly higher than those in the prospectus of the pharmaceutical. The relative standard deviations were below 5% for amoxicillin and below 7% for clavulanic acid. These results are acceptable because, to prevent degradation due to bacteriostatic activity, the concentration of the main reactant is usually higher.
More Related Videos
Related Concept Videos
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
Drug Accumulation During Multiple Dosing: Repetitive IV Injections
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation
On...
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses
One-Compartment Open Model for IV Bolus Administration: Estimation of Clearance
In the one-compartment open model for intravenous (IV) bolus administration, clearance is estimated by dividing the elimination rate by the plasma drug concentration. This equation leverages the elimination rate constant and the apparent...
Estimation of k and VD of Aminoglycosides

