Antibacterial activity of two adhesive systems using various microbiological methods.

Area of Science:

• Dental Materials Science
• Microbiology
• Biomaterials

Background:

• Dentin bonding systems (DBS) are crucial in restorative dentistry.
• Assessing the antibacterial properties of DBS is vital for preventing secondary caries.
• Limited comparative studies exist on the antimicrobial efficacy of different DBS.

Purpose of the Study:

• To compare the antibacterial activities of Clearfil Protect Bond and Xeno III.
• To evaluate DBS efficacy using agar well, paper disk, dentin disk, and cavity tooth models.
• To determine the most effective dentin bonding system for bacterial inhibition.

Main Methods:

• Agar well diffusion, paper disk diffusion, and dentin disk assays were performed with Streptococcus mutans.
• A human molar cavity model was infected with S. mutans and treated with DBS.
• Zones of inhibition and bacterial recovery counts were measured to assess antibacterial effects.

Main Results:

• Clearfil Protect Bond primer showed the largest zones of inhibition across all tested methods.
• Unpolymerized Xeno III also exhibited antibacterial activity, though less than Clearfil Protect Bond.
• Clearfil Protect Bond significantly reduced bacterial recovery in the tooth cavity model compared to Xeno III.

Conclusions:

• Clearfil Protect Bond exhibits the most potent antibacterial properties among the evaluated dentin bonding systems.
• Clearfil Protect Bond is more effective in inactivating bacteria within a tooth cavity than Xeno III.
• These findings support the use of Clearfil Protect Bond for its antimicrobial benefits in dental restorations.