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Quantification of faropenem in human plasma by high-performance liquid chromatography
Ramakrishna V S Nirogi1, Vishwottam N Kandikere, Wishu Shrivastava
1Biopharmaceutical Research, Suven Life Sciences Ltd, Hyderabad India. nvsrk@suven.com
Arzneimittel-Forschung
|January 25, 2006
Summary
A new high-performance liquid chromatography (HPLC) method accurately quantifies faropenem, a beta-lactam antimicrobial, in human plasma. This validated assay is suitable for pharmacokinetic studies.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Faropenem is a novel beta-lactam antimicrobial agent.
- Accurate quantification of drugs in biological matrices is crucial for pharmacokinetic studies.
Purpose of the Study:
- To develop and validate a simple, sensitive, and specific high-performance liquid chromatography (HPLC) method for quantifying faropenem in human plasma.
Main Methods:
- High-performance liquid chromatography (HPLC) with ultraviolet detection at 315 nm.
- Solid-phase extraction (SPE) for sample preparation.
- Isocratic mobile phase on a reverse-phase Waters symmetry C18 column.
Main Results:
- The method demonstrated a lower limit of quantitation of 200 ng/mL with high precision (<2% RSD).
- A wide linear range (200-25000 ng/mL) and excellent stability of faropenem in plasma were observed.
- No interference was detected from commonly coadministered drugs.
Conclusions:
- The validated HPLC method is sensitive, simple, and repeatable.
- This method is suitable for determining faropenem concentrations in human plasma for pharmacokinetic investigations.
