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Isolation and Characterization of Neutrophils with Anti-Tumor Properties
Published on: June 19, 2015
Loss of CD19 expression in B-cell neoplasms
N Masir1, T Marafioti, M Jones
1Leukaemia Research Fund Immunodiagnostics Unit, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
Histopathology
|January 25, 2006
Summary
An antibody can detect CD19 in routine biopsies, but its expression varies in lymphomas. CD19 is often absent in specific B-cell neoplasms and lymphocyte predominant Hodgkin's disease.
Area of Science:
- Immunohistochemistry
- Hematopathology
- Molecular diagnostics
Background:
- CD19 is a B-cell marker.
- Detecting CD19 in routine biopsies is crucial for lymphoma diagnosis.
Purpose of the Study:
- To evaluate an antibody for detecting CD19 in routine biopsy specimens.
- To detail CD19 expression in human lymphomas.
Main Methods:
- Immunostaining of paraffin-embedded normal and neoplastic lymphoid tissues using a polyclonal antibody to CD19's C terminus.
Main Results:
- CD19 widely expressed in normal B cells and extramedullary plasma cells.
- Variable CD19 expression in B-cell neoplasms (e.g., weak/negative in follicular and diffuse large B-cell lymphomas).
- Frequent absence of CD19 in classical Hodgkin's disease, post-transplant lymphoproliferative disease, plasma cell neoplasms, and lymphocyte predominant Hodgkin's disease.
Conclusions:
- CD19 is detectable in routine biopsy specimens.
- Unlike CD20, CD19 expression is not consistently strong in all B-cell neoplasms.
- Lymphocyte predominant Hodgkin's disease (L&H cells) commonly lacks CD19 expression.
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