Observation of the inverted hexagonal phase of lipids by scanning tunneling microscopy

Scanning tunneling microscopy (STM) has been used in direct observations of the inverted hexagonal phase of several lipids, such as cardiolipin (CL) in the presence of Ca2+, soybean PE, DOPE and a binary system of CL and DMPC as well. Single tube-like structures, bundles of tubes and the cross-sectional view of the HII phase can clearly be seen at room temperature in a near natural state of these lipids. The success of this study shed some light on the applicability of STM in the investigation of lipid polymorphism and its role in the function of biological membranes.