Related Experiment Video
Updated: Aug 13, 2026
04:11
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Genotyping of hepatitis B virus (HBV) by oligonucleotides microarray
Yajun Song1, Erhei Dai, Jin Wang
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Fengtai District, Beijing, People's Republic of China.
Molecular and Cellular Probes
|January 25, 2006
Summary
This study presents a novel oligonucleotide microarray for hepatitis B virus (HBV) genotyping. The developed microarray accurately identifies HBV genotypes in patient sera, offering a reliable and efficient diagnostic tool.
Area of Science:
- Virology
- Molecular Diagnostics
- Genetics
Background:
- Hepatitis B virus (HBV) genotyping is crucial for understanding disease epidemiology and guiding treatment.
- Oligonucleotide microarrays offer a potential platform for rapid and accurate genotyping.
Purpose of the Study:
- To develop and validate an oligonucleotide microarray for genotyping hepatitis B virus (HBV).
- To assess the performance of the microarray in clinical samples and determine genotype distribution.
Main Methods:
- Design of an oligonucleotide microarray with probes for HBV genotypes A-G and ape genotypes.
- Amplification and Cy5-dCTP labeling of HBV pre-S gene targets from patient serum samples.
- Hybridization of labeled PCR products to the microarray for genotyping.
Main Results:
- The microarray successfully genotyped 96 HBV patient serum samples, identifying 34 genotype B, 60 genotype C, and 2 B/C coinfections.
- Genotyping results showed 100% concordance with sequencing data for 24 randomly selected samples.
- Geographic analysis revealed distinct distributions for genotype C (Northern China) and genotype B (Southern China).
Conclusions:
- The developed low-density oligonucleotide microarray is a reliable and time-saving method for HBV genotyping.
- This method provides accurate and efficient genotyping directly from patient sera.
- The findings support the use of oligonucleotide microarrays in clinical virology diagnostics.
Related Concept Videos
01:25Hepatitis
Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
02:34DNA Microarrays
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...