Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Manometric transduction in enzyme biosensors.

Beatriz Fernandez Serra1, Achilles Tzoris, Elizabeth A H Hall

  • 1Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK.

Biosensors & Bioelectronics
|January 25, 2006
PubMed
Summary

This study presents a novel manometric biosensor using gas-evolving enzymes for analyte detection. Immobilized enzyme preparations and headspace gas analysis enable sensitive and reusable glucose monitoring.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Designing a locally produced DENV-1 nucleic acid diagnostic for low resource regions with endemic disease.

Analytical methods : advancing methods and applications·2025
Same author

Correction: Modifying recombinant purple acid phosphatase using computational design.

European biophysics journal : EBJ·2025
Same author

Modifying recombinant purple acid phosphatase using computational design.

European biophysics journal : EBJ·2025
Same author

Active diagnostic ingredients (ADIs) for PCR: A mini-bioreactor producing dNTPs with silica immobilized R5-kinases.

Biotechnology and bioengineering·2024
Same author

Biocatalytic synthesis of 2'-deoxynucleotide 5'-triphosphates from bacterial genomic DNA: Proof of principle.

Biotechnology and bioengineering·2023
Same author

Corrigendum: Electrochemically induced <i>in vitro</i> focal hypoxia in human neurons.

Frontiers in cell and developmental biology·2023

Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Enzyme Kinetics

Background:

  • Biosensors commonly utilize enzymatic recognition for analyte detection.
  • Manometric transduction offers a sensitive method for measuring gas production or consumption.
  • Integrating these approaches can lead to novel analytical devices.

Purpose of the Study:

  • To explore the combination of enzymatic recognition and manometric transduction for biosensing.
  • To develop a reusable biosensor system for analyte monitoring.
  • To investigate the factors influencing sensor performance, such as headspace and enzyme immobilization.

Main Methods:

  • Enzymes that produce or consume low-solubility gases were employed.
  • Analyte turnover was correlated with changes in headspace gas partial pressure.

Related Experiment Videos

  • A Teflon-enzyme composite was developed for enzyme immobilization.
  • A glucose oxidase system was used to monitor glucose consumption during fermentation.

    • Main Results:

    • The design relates headspace gas pressure changes to enzyme turnover.
    • Headspace and sample volume dimensions were analyzed for their impact on air-water interface flux.
    • Diffusion and reaction dynamics within the enzyme solution were elucidated.
    • Enzyme immobilization on a Teflon-enzyme composite enabled reusable preparations.
    • A disc with a stirring magnet proved to be an efficient configuration.

    Conclusions:

    • The developed manometric biosensor effectively monitors analyte turnover.
    • Enzyme immobilization strategies enhance reusability and sensor performance.
    • The system demonstrates potential for various enzyme-based sensing applications, including glucose monitoring.