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Neuropathogenesis of herpesvirus papio 2 in mice parallels infection with Cercopithecine herpesvirus 1 (B virus) in

Kristin M Rogers1, Jerry W Ritchey1, Mark Payton1

  • 1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078-2007, USA.

The Journal of General Virology
|January 25, 2006
PubMed

Insights

Cercopithecine herpesvirus 16 (HVP2) shows neurovirulence in mice, mirroring human monkey B virus (BV) infections. This mouse model can help study the severe zoonotic BV infections.

Area of Science:

  • Virology
  • Neuroscience
  • Zoonotic Diseases

Background:

  • Cercopithecine herpesvirus 1 (monkey B virus; BV) causes severe, often fatal, human infections.
  • Cercopithecine herpesvirus 16 (HVP2), closely related to BV, has unknown human infection cases.
  • HVP2 causes fatal central nervous system (CNS) infections in mice after peripheral inoculation.

Purpose of the Study:

  • Evaluate HVP2 neurovirulence in mice as a model for human BV infections.
  • Investigate viral determinants of zoonotic BV infection severity.

Main Methods:

  • Mice were inoculated with HVP2 via epidermal scarification, intracranial inoculation, and eye splash.
  • Quantitative real-time PCR, histopathology, and immunohistochemistry tracked viral spread.
  • Neurovirulent and apathogenic HVP2 isolates were compared.

Main Results:

  • HVP2 was consistently neurovirulent across different inoculation routes.
  • Apathogenic HVP2 isolates reached the CNS but replicated inefficiently.
  • Neurovirulent HVP2 strains demonstrated a pathogenesis in mice similar to human BV infections.

Conclusions:

  • Neurovirulent HVP2 strains in mice provide a relevant model for studying human BV infections.
  • This model system aids in dissecting viral factors contributing to BV's zoonotic severity.

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