Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A versatile tool for conditional gene expression and knockdown.

Jolanta Szulc1, Maciej Wiznerowicz, Marc-Olivier Sauvain

  • 1School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

Nature Methods
|January 25, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tissue-specific restriction of transposon-derived regulatory elements safeguards cell-type identity.

Cell reports·2026
Same author

Identification of a lipid oxygen radical defense pathway and its epigenetic control.

Nature communications·2025
Same author

Mix-and-match between transposable elements and zinc finger proteins fuels genic and regulatory innovation.

Current opinion in genetics & development·2025
Same author

The 'mono-side' of cutaneous adverse drug reactions.

Journal of the European Academy of Dermatology and Venereology : JEADV·2025
Same author

Cancer cells subvert the primate-specific KRAB zinc finger protein ZNF93 to control APOBEC3B.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Immunohistological Examination of HEATR1 and SLC27A2 Expression in ccRCC Samples to Evaluate Their Potential as Prognostic Markers-A Preliminary Study.

Cancers·2025
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026
See all related articles

We developed a novel drug-controllable gene expression system for mammalian cells. This robust lentiviral vector system offers precise control for genetic research and gene therapy applications.

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Mammalian Genetics

Background:

  • Drug-inducible systems are crucial for controlling gene expression in mammalian cells for research and therapeutics.
  • Existing systems often suffer from limitations like leakiness, low induction, lack of specificity, or complex designs, hindering in vivo applications.

Purpose of the Study:

  • To develop and characterize a versatile, drug-controllable lentiviral vector-based gene expression system.
  • To enable precise regulation of transgenes or small inhibitory hairpin RNAs (shRNAs) using a pharmaceutical compound.

Main Methods:

  • Construction and utilization of a lentiviral vector system for conditional gene expression.
  • Testing the system's efficacy in various mammalian cell types and in vivo models, including cell lines, stem cells, xenotransplanted tumors, and transgenic mice.

Related Experiment Videos

Main Results:

  • Demonstrated robust and tightly controlled gene expression across diverse models, including cell lines, stem cells, and in vivo systems.
  • Successfully regulated both polymerase (Pol) II promoter-driven transgenes and Pol III promoter-controlled shRNAs.
  • Showcased the system's versatility in human tumors xenotransplanted into mice, rat brains, and transgenic mice.

Conclusions:

  • The developed lentiviral vector-based system provides a reliable and versatile tool for drug-controllable gene expression in mammalian systems.
  • This system holds significant promise for advancing basic genetic research and developing novel gene-based therapeutics.