Modulation of visual inputs to accessory optic system by theophylline during hypoxia

Neural tissues from fresh water turtles have been electrophysiologically studied in vitro due to their significant resistance to hypoxia. Such neurons have resting membrane potentials that are similar to intact animals and receive similar synaptic inputs evoked by sensory stimuli. One mechanism to reduce the brain's metabolic requirement in the absence of oxygenated blood flow was investigated by blocking adenosine receptors before and during hypoxia. Extracellular and whole-cell patch recordings were made from the basal optic nucleus, whose neurons respond to visual stimuli in vitro. While the addition of the adenosine antagonist theophylline to oxygenated superfusate had minimal effect on the neural activity, theophylline in superfusate bubbled with nitrogen strongly increased activity compared to either oxygenated theophylline or control superfusate bubbled with nitrogen. The increase in spontaneous activity was due to increases to both amplitude and frequency of excitatory synaptic events. Even during these increases, the neurons continued to exhibit their direction-sensitive responses. These results indicate that adenosine may play a role in protecting the viability of the brainstem during hypoxia without reducing visually mediated brainstem reflex control.