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Related Experiment Videos

Cellular prion protein is released on exosomes from activated platelets.

Catherine Robertson1, Stephanie A Booth, Daniel R Beniac

  • 1Department of Oral Biology, University of Manitoba, 780 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0W2.

Blood
|January 26, 2006
PubMed
Summary

Cellular prion protein (PrP(C)) is found in platelets. Upon activation, platelets release PrP(C) on microvesicles and exosomes, suggesting a role in cell-to-cell transmission.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Neuroscience

Background:

  • Cellular prion protein (PrP(C)) is a GPI-anchored protein with an unknown function.
  • PrP(C) is abundant in human platelets, the largest reservoir in blood.
  • Misfolded PrP(Sc) is implicated in transmissible spongiform encephalopathies (TSEs), and PrP(C) presence is crucial for disease progression.

Purpose of the Study:

  • To investigate the location and behavior of PrP(C) in blood, particularly in platelets.
  • To understand the physiological function of PrP(C) by examining its distribution in blood components.
  • To explore the potential role of PrP(C) in cell-to-cell transmission.

Main Methods:

  • Investigated PrP(C) localization on resting and activated platelets.
  • Analyzed the release of PrP(C) on platelet-derived microvesicles and exosomes.

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  • Utilized techniques to detect and characterize PrP(C) expression and release.

    • Main Results:

    • PrP(C) is located on the alpha granule membrane of resting platelets.
    • Platelet activation induces transient surface expression of PrP(C).
    • Activated platelets release PrP(C) on both microvesicles and exosomes.

    Conclusions:

    • Platelet activation leads to the release of PrP(C) via microvesicles and exosomes.
    • PrP(C) on platelet-derived exosomes may facilitate its transport in the bloodstream.
    • This finding suggests a potential mechanism for PrP(C) mediated cell-to-cell transmission.