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Updated: Aug 13, 2026
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its
Hong-Cui Cao1, Xiao-Ming Chen, Wei Xu
1Department of Infectious Disease, First Affiliated Hospital, Medical School of Zhejiang University, Zhejiang Province, China.
Aim:
To detect the platelet-activating factor (PAF) and the plasma or serum levels of tumor necrosis factor-alpha (TNF-alpha) malondialdehyde (MDA), endotoxin (ET) and to discuss their significance in various types of viral hepatitis.
Methods:
PAF, TNF-alpha, MDA, and ET levels in 60 controls, 16 cases of acute viral hepatitis, 71 cases of chronic viral hepatitis, 19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography (rHPLC), bio-assay, ELISA, thiobarbituric acid (TBA), and limulus lysate test (LLT), respectively.
Results:
The rHPLC was more sensitive and specific than bio-assay (r = 0.912, P<0.01). The plasma levels of PAF, TNF-alpha, MDA, and ET in patients with viral hepatitis were higher than those in controls (P<0.01).
Conclusion:
rHPLC is more reliable and accurate for the detection of PAF.
