Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Direct interaction between cohesin complex and DNA replication machinery.

Min-Jung Ryu1, Beom-Jun Kim, Jeong-Won Lee

  • 1Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300 Chonchon-Dong, Suwon, Kyunggi-Do 440-746, Republic of Korea.

Biochemical and Biophysical Research Communications
|January 28, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Romosozumab and Cardiovascular Safety: Bridging the Gap between Clinical Trials and Real-World Evidence.

Endocrinology and metabolism (Seoul, Korea)·2026
Same author

Elevated Serum CXCL9 Levels Are Associated with Greater Frailty Risk in Older Women.

Endocrinology and metabolism (Seoul, Korea)·2026
Same author

Longitudinal multi-platform profiling reveals temporal dynamics of HER2, TROP2, PD-L1 and tumor-infiltrating lymphocytes in triple-negative breast cancer.

medRxiv : the preprint server for health sciences·2026
Same author

Elevated Circulating Ceramides 18:0 and 24:1 as a Risk Factor for Sarcopenia: In Vitro, Animal, and Clinical Evidence.

Journal of cachexia, sarcopenia and muscle·2026
Same author

Nationwide Big Data Studies of Endocrine Diseases Using the Korean National Health Information Database: Research Trends and Standardization of Operational Definitions.

Endocrinology and metabolism (Seoul, Korea)·2026
Same author

Sarcopenia and Muscle Aging: Updated Insights into Molecular Mechanisms and Translational Therapeutics.

Endocrinology and metabolism (Seoul, Korea)·2026
Same journal

NCF1 aggravates cerebral ischemia-reperfusion injury by amplifying NOX2-Dependent oxidative stress and inflammatory responses.

Biochemical and biophysical research communications·2026
Same journal

Functional and structural characterization of Akkermansia muciniphila thioredoxin domain-containing protein Amuc_2173.

Biochemical and biophysical research communications·2026
Same journal

SMURF1-mediated EFEMP1 ubiquitination reverses the resistance of HCC cells to sorafenib by promoting ferroptosis.

Biochemical and biophysical research communications·2026
Same journal

Development and validation of HSP90AA1 as a risk gene in a HIF-1α pathway-related prognostic signature for hepatocellular carcinoma.

Biochemical and biophysical research communications·2026
Same journal

Quercetin suppresses TGF-β1-induced proliferation and migration of vascular smooth muscle cells via the Smad2/3/MMP-9 signaling axis.

Biochemical and biophysical research communications·2026
Same journal

Biosynthesis, characterization and biological potential of microbe-mediated silver nanoparticles using thermophilic actinomycetes, Streptomyces nigra.

Biochemical and biophysical research communications·2026
See all related articles

Structural maintenance of chromosome 1 (Smc1) interacts with DNA replication proteins, including minichromosome maintenance 7 (Mcm7). This suggests a functional link between the cohesin complex and DNA replication machinery.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Structural maintenance of chromosome 1 (Smc1) is crucial for DNA repair and cell cycle checkpoint activation.
  • Understanding Smc1's interactions is key to elucidating its diverse cellular roles.

Purpose of the Study:

  • To identify novel proteins interacting with Smc1.
  • To investigate the functional relationship between Smc1 and DNA replication proteins.

Main Methods:

  • Tandem affinity purification (TAP) coupled with MALDI-TOF mass spectrometry to identify Smc1-interacting proteins.
  • Co-immunoprecipitation and GST pull-down assays to confirm and characterize protein interactions.

Main Results:

  • Minichromosome maintenance 7 (Mcm7), a pre-replication complex component, was identified as a novel Smc1-interacting protein.

Related Experiment Videos

  • Physical interaction between Smc1 and Mcm7 was confirmed in vitro and in vivo, with specific interaction domains identified.
  • Smc1 was also found to interact with other DNA replication factors, such as Mcm6, RFC1, and DNA polymerase alpha.

    • Conclusions:

    • A functional link between the cohesin complex (represented by Smc1) and DNA replication proteins is suggested.
    • These findings open new avenues for research into the coordination of chromosome structure and DNA replication.