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P-selectin- and CD63-exposing platelet microparticles reflect platelet activation in peripheral arterial disease and

P Marc van der Zee1, Eva Biró, Yung Ko

  • 1Department of Cardiology and Laboratory of Experimental Clinical Chemistry, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands. p.m.vanderzee@amc.uva.nl

Clinical Chemistry
|January 28, 2006
PubMed
Abstract

Insights

Platelet-derived microparticles (PMPs) subpopulations, not total numbers, better indicate platelet activation. Circulating PMP subpopulations increase with aging, peripheral arterial disease, and myocardial infarction.

Area of Science:

  • Cardiovascular Medicine
  • Hematology
  • Biomarker Discovery

Background:

  • Platelet-derived microparticles (PMPs) are recognized markers of platelet activation in cardiovascular disease.
  • The relationship between specific PMP subpopulations and in vivo platelet activation requires further elucidation.

Purpose of the Study:

  • To investigate whether PMP subpopulations correlate with platelet activation markers in vitro.
  • To assess circulating PMP subpopulations in patients with various cardiovascular conditions compared to healthy controls.

Main Methods:

  • Flow cytometry was used to analyze PMP subpopulations from resting and activated platelets in vitro.
  • Plasma samples from patients with stable angina, peripheral arterial disease, myocardial infarction (NSTEMI, STEMI), and healthy individuals were analyzed.
  • Coagulation markers (prothrombin fragment F(1+2), thrombin-antithrombin complexes) and soluble P-selectin were measured.

Main Results:

  • In vitro, platelet activation increased PMPs expressing P-selectin or CD63.
  • Circulating P-selectin-exposing PMPs were elevated in older individuals and patients with NSTEMI and STEMI.
  • CD63-exposing PMPs increased in patients with peripheral arterial disease, NSTEMI, and STEMI.

Conclusions:

  • PMP subpopulations serve as more accurate indicators of platelet activation than total PMP counts.
  • Elevated circulating PMP subpopulations are associated with aging, peripheral arterial disease, and myocardial infarction.

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