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Temporal differences in bladder dysfunction caused by diabetes, diuresis, and treated diabetes in mice
Firouz Daneshgari1, Xiao Huang, Guiming Liu
1Glickman Urological Institute, and Department of Quantitative Health Sciences, Cleveland Clinic Foundation, 9500 Euclid Ave., ND50, Cleveland, OH 44195, USA. daneshf@ccf.org
Summary
Diabetic bladder dysfunction progresses over time in mice, transitioning from compensated to decompensated states around 9-12 weeks after diabetes onset. Insulin therapy effectively reverses these detrimental bladder changes.
Area of Science:
- Urology
- Endocrinology
- Diabetology
Background:
- Diabetic bladder dysfunction is a frequent complication of diabetes mellitus (DM).
- The natural history and temporal progression of diabetic bladder dysfunction remain poorly understood.
- Understanding these changes is crucial for managing diabetic patients.
Purpose of the Study:
- To investigate the temporal changes in bladder function following diabetes induction in mice.
- To compare diabetic bladder dysfunction with diuretic-induced changes and insulin-treated diabetic mice.
- To identify the time course of bladder decompensation in diabetes.
Main Methods:
- Streptozotocin (STZ)-induced diabetes in male C57BL/6 mice.
- Conscious cystometrograms and micturition cycle measurements.
- Comparison groups included insulin-treated diabetic mice, sucrose-induced diuretic mice, and sham controls.
Main Results:
- Diabetes and diuresis increased bladder weight, urine output, capacity, and compliance.
- Peak voiding pressure initially increased but then dropped significantly in diabetic mice after 12 weeks.
- Bladder emptying declined significantly in diabetic mice after 12 weeks, unlike in diuretic mice.
- Insulin treatment reversed most observed bladder function changes.
Conclusions:
- Diabetic bladder dysfunction progresses through distinct phases.
- A transition from compensated to decompensated bladder dysfunction occurs approximately 9-12 weeks after STZ-induced diabetes onset in mice.
- Long-term insulin therapy can ameliorate diabetic bladder dysfunction.
