Myosin heavy chain isoform expression: influence on isoinertial and isometric performance

Thirty-six healthy men with varying degrees of physical training background performed maximal-effort isometric and isoinertial knee extensor actions, with relative loads equal to 40% and 70% of one-repetition maximum. Force, velocity, and power were derived from force and linear position transducers at 500 Hz. Biopsies were taken from the vastus lateralis and analyzed by SDS-PAGE for relative myosin heavy chain (MHC) content. Relative MHC IIx content was included in a regression model, and explained variance noted. Relative MHC I content was subsequently added to the regression model to determine what, if any, additional variance was explained beyond that of MHC IIx. Results indicated that no relationship (r = 0.0 to 0.1) exists between the relative expression of MHC isoforms from the vastus lateralis and isometric/isoinertial performance in a population with diverse training backgrounds. Lack of nervous system adaptations in the untrained subjects in the study possibly attenuates the significant relationship between MHC and in-vivo muscle performance previously established in trained populations.