Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Resonant nano-cluster devices.

J Haglmüller1, H Rauter, G Bauer

  • 1Institute of Biochemistry and Molecular Cell Biology, University of Vienna, Austria.

IEE Proceedings. Nanobiotechnology
|January 31, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immobilization ofmyo-inositol-1-phosphate synthase containing active, self-regenerating coenzyme (NAD(+)) on the same matrix.

Applied biochemistry and biotechnology·2013
Same author

Influence of urea and organic solvents on the activity of immobilizedmyo-inositol-1-phosphate synthase containing active, self-regenerating coenzyme (NAD(+)) on the same matrix.

Applied biochemistry and biotechnology·2013
Same author

Temperature-dependent behavior of immobilized alkaline phosphatase. I. Role of working conditions and pH during coupling.

Applied biochemistry and biotechnology·2013
Same author

Temperature-dependent behavior of immobilized alkaline phosphatase II. temperature pulse : A tool to enhance enzymatic activity for prolonged periods.

Applied biochemistry and biotechnology·2013
Same author

Highly sensitive determination of DAO activity by oxidation of a luminescence reagent.

Applied biochemistry and biotechnology·2007
Same author

Novel transducers for nano-optical biosensor chips based on biological and synthetic polymers with analyte-dependent swelling/shrinking behavior.

Journal of nanoscience and nanotechnology·2004
Same journal

Dynamics of rotating paramagnetic particles simulated by lattice Boltzmann and particle dynamics methods.

IEE proceedings. Nanobiotechnology·2006
Same journal

Absorption detection of enzymatic reaction using optical microfluidics based intermittent flow microreactor system.

IEE proceedings. Nanobiotechnology·2006
Same journal

Analysis of cellular structure by light scattering measurements in a new cytometer design based on a liquid-core waveguide.

IEE proceedings. Nanobiotechnology·2006
Same journal

Application of nano-indenter for investigation of the properties of the elytra cuticle of the dung beetle (Copris ochus Motschulsky).

IEE proceedings. Nanobiotechnology·2006
Same journal

Characterisation of pore structures in nanoporous materials for advanced bionanotechnology.

IEE proceedings. Nanobiotechnology·2006
Same journal

Freestanding polyelectrolyte multilayers as functional and construction elements.

IEE proceedings. Nanobiotechnology·2006
See all related articles

Resonance-enhanced absorption (REA) bio-optical devices utilize metal nano-clusters for sensitive detection. Optimized lab protocols enable stable, high-performance biochips for various biomolecular interactions.

Area of Science:

  • Biophotonics
  • Nanotechnology
  • Biosensing

Background:

  • Resonance-enhanced absorption (REA) using surface-bound metal clusters offers a promising foundation for bio-optical devices.
  • Existing methods using spherical gold colloids face spectral limitations.

Purpose of the Study:

  • To develop and present optimized lab protocols for biochips utilizing the REA process.
  • To investigate the influence of resonator homogeneity and layer stability on device performance.
  • To establish versatile biochip platforms for various biomolecular detection systems.

Main Methods:

  • Fabrication of a four-layer device including a metal mirror, distance layer, biomolecule interaction layer, and metal nano-clusters.
  • Synthesis of metal clusters and metal-dielectric shell clusters for precise spectral tuning.

Related Experiment Videos

  • Development of optimized laboratory protocols for biochip assembly and REA detection.

    • Main Results:

    • Demonstrated strong influence of resonator homogeneity on the absorption maximum.
    • Highlighted the critical role of layer stability in overall device performance.
    • Achieved sensors with narrow reflection minima in the visible and IR spectrum, overcoming limitations of spherical colloids.
    • Successfully developed disposable protein chips, DNA assays, and complex biochip arrays.

    Conclusions:

    • The developed REA-based biochip platform offers high performance and spectral versatility.
    • Optimized protocols and materials enable stable and reliable detection of various biomolecular interactions.
    • This technology provides a robust foundation for advanced bio-optical devices and diagnostics.