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Infrared up-converting phosphors for bioassays.

P L A M Corstjens1, S Li, M Zuiderwijk

  • 1Leiden University Medical Center, Leiden, The Netherlands. corstjens@lumc.nl

IEE Proceedings. Nanobiotechnology
|January 31, 2006
PubMed
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Up-converting phosphor reporter particles offer a stable, sensitive alternative to conventional fluorescent reporters. Their infrared up-conversion property enables permanent, bleach-free excitation for advanced bioassays.

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Up-converting phosphor reporter particles represent an advancement in detection technologies.
  • These particles feature core-shell structures with functional groups for bio-conjugation, offering chemical stability and infrared up-conversion properties.
  • Unlike conventional fluorescent reporters, they do not bleach and allow for permanent excitation with signal integration.

Purpose of the Study:

  • To review the production and characteristics of up-converting phosphor technology (UPT) reporter particles.
  • To highlight the unique properties of UPT for highly-sensitive particle-based assays.
  • To discuss the application of UPT in various bioassays.

Main Methods:

  • Review of literature on the synthesis and characterization of up-converting phosphor reporter particles.

Related Experiment Videos

  • Analysis of the optical properties, including infrared up-conversion and large anti-Stokes shift.
  • Examination of UPT applications in diverse biological detection systems.

    • Main Results:

    • UPT reporter particles exhibit chemical stability and unique infrared up-conversion capabilities.
    • A large anti-Stokes shift (>500 nm) ensures distinct emission from excitation sources.
    • Absence of autofluorescence under infrared excitation provides unmatched contrast in biological specimens.

    Conclusions:

    • Up-converting phosphor technology offers significant advantages for sensitive particle-based bioassays.
    • The non-bleaching nature and high contrast make UPT a powerful tool for advanced detection.
    • UPT reporter particles are versatile for a wide range of bioassay applications.