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Updated: Aug 13, 2026

Cryo-EM and Single-Particle Analysis with Scipion
Published on: May 29, 2021
Cryo-EM and single particles
1Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA.
Abstract:
Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.
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