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Updated: Aug 9, 2026

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
Published on: December 17, 2013
Pathfinders and trailblazers: a prokaryotic targeting system for transport of folded proteins
Frank Sargent1, Ben C Berks, Tracy Palmer
1School of Biological Sciences, University of East Anglia, Norwich, UK. f.sargent@uae.ac.uk
Abstract:
The twin-arginine (Tat) protein translocase is a highly unusual protein transport machine that is dedicated to the movement of folded proteins across the bacterial cytoplasmic membrane. Proteins are targeted to the Tat pathway by means of N-terminal signal peptides harbouring a distinctive twin-arginine motif. In this minireview, we describe our current knowledge of the Tat system, paying particular attention to the function of the TatA protein and to the often overlooked step of signal peptide cleavage.
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