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Interaction analysis of the AAA ATPase TAA43 by the bacterial two-hybrid system
1Division of Molecular Biology, Institute for Scientific and Technological Research of San Luis Potosi, San Luis Potosi, Mexico. lsantos@ipicyt.edu.mx
Abstract:
The TAA43 ATPase of Thermoplasma acidophilum, an archaeal member of the AAA protein family, is known to have an atypical oligomeric state and a nonspecific association with high-molecular-weight protein complexes. We assessed the in vivo binding pattern of TAA43 using the bacterial two-hybrid system. We found 36 positive isolates interacting with TAA43. Our analysis showed that TAA43 interacts preferentially with nonribosomal proteins containing ribosomal domains and regions involved in RNA metabolism.
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