Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intercellular adhesion molecule-1 expression in human endometrium: implications for long term progestin only

Frederick Schatz1, Graciela Krikun, Rebecca N Baergen

  • 1Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA. frederick.schatz@yale.edu

Reproductive Biology and Endocrinology : RB&E
|February 1, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Letter to the editor: Anovulatory bleeding and the spectrum of bleeding disorders: Understanding heavy menstrual bleeding in adolescents.

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2026
Same author

Appraisal of international, patient-facing resources on heavy menstrual bleeding.

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2026
Same author

The Jacobins of medicine: how ideological zeal imperils professional integrity.

The American journal of medicine·2025
Same author

The potential bidirectional relationship between long COVID and menstruation.

Nature communications·2025
Same author

Active chromatin marks and up-regulation of FOXC1 in uterine epithelial cells demarcate the onset of reproductive decline in aging females.

NAR molecular medicine·2025
Same author

Advancing physician-scientist career development in obstetrics and gynecology: 40 years of the American Association of Obstetricians and Gynecologists Foundation scholar program.

American journal of obstetrics and gynecology·2025

Intercellular adhesion molecule-1 (ICAM-1) expression in the uterine lining does not change during the menstrual cycle or with long-term progestin-only contraceptive use. This suggests other factors influence neutrophil influx into the endometrium.

Area of Science:

  • Reproductive biology
  • Immunology
  • Gynecology

Background:

  • Neutrophils accumulate in the endometrium during the premenstrual phase and with long-term progestin-only contraceptive (LTPOC) use.
  • Neutrophil migration is mediated by intercellular adhesion molecule-1 (ICAM-1) on endothelial cells.
  • Previous research on ICAM-1 expression in the endometrium yielded conflicting results regarding cell type specificity and cyclic variation.

Purpose of the Study:

  • To investigate the expression patterns of ICAM-1 in the endometrium across the menstrual cycle and during LTPOC treatment.
  • To determine if ICAM-1 expression by endothelial cells contributes to neutrophil infiltration in the endometrium.

Main Methods:

  • Endometrial biopsies were collected from women throughout their menstrual cycles and from those using LTPOCs (Mirena, Norplant).

Related Experiment Videos

  • Tissues were processed using formalin fixation and paraffin embedding.
  • Immunohistochemical staining was performed to detect ICAM-1 expression.

    • Main Results:

    • Consistent and prominent ICAM-1 staining was observed in endometrial endothelial cells.
    • ICAM-1 expression intensity in blood vessels remained uniform regardless of hormonal status or inflammation across the menstrual cycle and during LTPOC therapy.
    • Glands showed no ICAM-1 staining, while stromal cells exhibited weak positive staining.

    Conclusions:

    • Altered endothelial ICAM-1 expression does not appear to be the cause of neutrophil infiltration in the premenstrual or LTPOC-treated endometrium.
    • The influx of neutrophils into the endometrium may be regulated by changes in neutrophil chemoattractant expression.