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Updated: Aug 13, 2026

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Synthesis and evaluation of NO-release from symmetrically substituted furoxans
William F Nirode1, Jessica M Luis, Jordan F Wicker
1Department of Chemistry, Hofstra University, Hempstead, NY 11549, USA. chmwfn@hofstra.edu
Abstract:
A series of symmetrically substituted dibenzoyl furoxans were synthesized and investigated for their potential to release nitric oxide, which plays a key role in the nervous and cardiovascular systems. Cysteine was employed to promote nitric oxide release from furoxan via the formation of an S-nitrosothiol intermediate. Transition metal ion-mediated S-nitrosocysteine decomposition liberates nitric oxide that, in aqueous aerobic solutions, is converted to reactive nitrogen oxide species. The percent nitric oxide released was quantified colorimetrically by the Griess reagent system.
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