Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[An evaluation method for organ preservation].

V Mirkovitch1, B Winistörfer

  • 1Division de chirurgie expérimentale, CHUV, Lausanne.

Helvetica Chirurgica Acta
|May 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mid-term results with cultured epidermal autografts, allogenic skin transplants and cyclosporin A medication.

Burns : journal of the International Society for Burn Injuries·1994
Same author

[Preservation of hepatocytes of the rat by active transport of glycine].

Helvetica chirurgica acta·1993
Same author

[Value of antral mucosectomy, selective proximal vagotomy and a combination of the 2 procedures in serum gastrin production and inhibition of stomach acid. An experimental study].

Helvetica chirurgica acta·1993
Same author

Alanine uptake by the endothelium of canine blood vessels and by the vessels of human umbilical cord.

European journal of vascular surgery·1992
Same author

[Development of a gastroplasty with variable diameter. Experimental study using artificial sphincters].

Helvetica chirurgica acta·1992
Same author

[Origin of secretions after denervation of the small intestine in the dog].

Helvetica chirurgica acta·1992

The University of Wisconsin (UW) solution best preserved canine endothelial cell and renal cortex slice viability during hypothermic storage. This solution maintained cellular function, showing superior protection over other preservation solutions tested.

Area of Science:

  • Cell Biology
  • Organ Preservation
  • Transplantation Research

Background:

  • Assessing cellular viability is crucial for evaluating organ preservation solutions.
  • Endothelial cells and renal cortex slices are key indicators of tissue health post-conservation.

Purpose of the Study:

  • To evaluate the efficacy of different preservation solutions in maintaining canine endothelial cell viability.
  • To compare the protective effects of Krebs bicarbonate buffer (KB), St. Thomas (ST), Euro-Collins (EC), and University of Wisconsin (UW) solutions.

Main Methods:

  • Canine endothelial cells and dog renal cortex slices were stored in vitro at 4°C.
  • Cellular viability was assessed by measuring the active transport of alanine.
  • Evaluations were performed after 24 and 72 hours of conservation.

Related Experiment Videos

Main Results:

  • The University of Wisconsin (UW) solution demonstrated the highest active transport values, indicating superior cellular viability.
  • UW solution provided the best protection for both endothelial cells and renal cortex slices.
  • This protective effect was consistent even after extended conservation periods of up to 72 hours.

Conclusions:

  • The University of Wisconsin (UW) solution is highly effective in preserving canine endothelial cell and renal cortex viability during hypothermic storage.
  • UW solution offers superior protection compared to KB, ST, and EC solutions for short-term and extended organ preservation.