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[Study on effects of salidroside on lipid peroxidation on oxidative stress in rat hepatic stellate cells]
1The South China University of Technology Hospital, Guangzhou, 510640.
Objective:
To investigate the effect of antioxidant Salidroside on the lipid peroxidation of oxidative stress in rat hepatic stellate cells in vitro.
Methods:
HSC-T6 were incubated with 0.1 mM ferric nitrilotriacetate complex (FeNTA). MTT colorimetry was used for assaying proliferation of HSC. Cytotoxicity was measured by LDH colorimetry. Collagen type I accummulation in the culture media was measured by ELISA. Intracellular MDA, SOD, GSH and GSH-PX levels in the culture media were measured by their reagent boxes.
Results:
HSC incubation with FeNTA resulted in a significant production of intracellular MDA and GSH, associated with decreasing SOD and GSH-PX activity. Exposure of HSC to FeNTA significantly enhanced the number of proliferating HSC and collagen type I levels in the culture medium. All these effects were reversed by the antioxidant Salidroside.
Conclusion:
The mechanism of Salidroside on fibrosis may be related to inhibit lipid peroxidation.